We conclude that the COL1A1 Sp1 polymorphism is a functional genetic variant that predisposes to osteoporosis by complex mechanisms involving changes in bone mass and bone quality. Finally, the yield strength of bone derived from “Ss” individuals was reduced when compared with bone derived from “SS” subjects. If the R 2 value is <0.9, we recommend repeating the experiment for greater reproducibility or in a different concentration range. A R 2 value of 0.9 is desirable for confidence in the results (see Note 21). Collagen produced from osteoblasts cultured from “Ss” heterozygotes had an increased ratio of α1(I) protein relative to α2(I), and this was accompanied by an increased ratio of COL1A1 mRNA relative to COL1A2. GraphPad Prism will generate a table of results which includes the calculated IC 50 value and the R 2 value for the data set (Fig. Gel shift assays showed increased binding affinity of the “s” allele for Sp1 protein, and primary RNA transcripts derived from the “s” allele were approximately three times more abundant than “S” allele–derived transcripts in “Ss” heterozygotes. Additionally, when a Prism project is opened from within your Notebook, it may be re-saved into the same Page from which is resides, preserving both versions of the file. When citing one of these pages, reference the name of the page, the date you accessed it and the URL. GraphPad Prism (Version 5.04 / 5.0d and higher) has been integrated with LabArchives to enable users to directly export projects from Prism into the LabArchives Notebook. UCSD Prism agreement renewal starts the first week of February 2023. Fund expiration date Well quote the price once we receive your software form request. The association with fracture was stronger than expected on the basis of the observed differences in BMD and BMI, suggesting an additional effect on bone strength. How to cite these pages - GraphPad Prism 9 Statistics Guide. Full name and UCSD email address of user/s who need a license 2. A meta-analysis showed significant associations between COL1A1 “s” alleles and bone mineral density (BMD), body mass index (BMI), and osteoporotic fractures. Here we explore the molecular mechanisms underlying this association. We previously described a polymorphic Sp1 binding site in the COL1A1 gene that has been associated with osteoporosis in several populations. Osteoporosis is a common disease with a strong genetic component.
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